Analysis Note

Positive ControlHeLa Cell Nuclear Extract (Cat. No. WB64)

Application

Immunoblotting (1:3000-1:5000)r>Immunofluorescence (1:300-1:500, indirect)r>Immunoprecipitation (1:300-1:500)

General description

This Anti-p53 Binding Protein 1 (Ab-1) Rabbit pAb is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of p53 Binding Protein 1 (Ab-1).

Purified rabbit polyclonal antibody. Recognizes the ~230 kDa 53BP1 protein.

Recognizes the ~230 kDa 53BP1 protein in HeLa cell nuclear extract (Cat. No. WB64).

Immunogen

a recombinant protein consisting of human 53BP1 fused to a His Tag™ sequence

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Antibody should be titrated for optimal results in individual systems.

Packaging

100 µL in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM NaCl, 50 mM Tris-HCl, 20% glycerol, pH 7.6.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Standard Handling (A)